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J Chromatogr A. 2012 Jan 6;1219:104-13. doi: 10.1016/j.chroma.2011.11.009. Epub 2011 Nov 10.

Simultaneous determination of thirty non-steroidal anti-inflammatory drug residues in swine muscle by ultra-high-performance liquid chromatography with tandem mass spectrometry.

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  • 1College of Veterinary Medicine, China Agricultural University, Beijing, China.

Abstract

An ultra-high-performance liquid chromatography with tandem mass spectrometric detection (UHPLC-MS/MS) method was established for the simultaneous determination of residues of thirty non-steroidal anti-inflammatory drugs (NSAIDs) in swine muscle. The samples were extracted with acetonitrile and phosphoric acid. The extracts were defatted with n-hexane, and then purified by HLB solid-phase extraction cartridge. Analysis was carried out on UHPLC-ESI-MS/MS working with multiple reaction monitoring mode with polarity switching. Limits of detection were between 0.4 μg/kg and 2.0 μg/kg, and limits of quantification were between 1.0 μg/kg and 5.0 μg/kg. The recoveries of NSAIDs were between 61.7% and 125.7% at spiked levels of 1.0-500 μg/kg. The repeatability was less than 8% and the within-laboratory reproducibility was not more than 12.3%. The method was reliable, convenient and sensitive.

Copyright © 2011 Elsevier B.V. All rights reserved.

PMID:
22142970
[PubMed - indexed for MEDLINE]
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