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Appl Environ Microbiol. 2012 Feb;78(3):892-4. doi: 10.1128/AEM.06580-11. Epub 2011 Dec 2.

Cultivated single-stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA-binding stains.

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  • 1Department of Natural Sciences, Linneaus University, Kalmar, Sweden. k.holmfeldt@gmail.com

Abstract

This is the first description of cultivated icosahedral single-stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA-binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.

PMID:
22138992
[PubMed - indexed for MEDLINE]
PMCID:
PMC3264134
Free PMC Article
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