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J Assist Reprod Genet. 2011 Dec;28(12):1171-6. doi: 10.1007/s10815-011-9678-6. Epub 2011 Nov 30.

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group.

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  • 1Reproductive Services, Royal Women's Hospital, Grattan Street & Flemington Road, Parkville, Victoria, 3052, Australia. debra.gook@mivf.com.au


Previous reports of slow cooling of human mature oocytes have shown a reduced clinical efficiency relative to fresh oocytes. This study reports that equivalent fertilization and implantation rates to those obtained using fresh oocytes and cryopreserved embryos can be achieved with human mature oocytes dehydrated in 1.5 M propanediol and 0.2 M sucrose at 37°C and cryopreserved using slow cooling rates.

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