Format

Send to:

Choose Destination
See comment in PubMed Commons below
PLoS One. 2011;6(11):e27472. doi: 10.1371/journal.pone.0027472. Epub 2011 Nov 17.

Crosstalk between ROS homeostasis and secondary metabolism in S. natalensis ATCC 27448: modulation of pimaricin production by intracellular ROS.

Author information

  • 1Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.

Abstract

Streptomyces secondary metabolism is strongly affected by oxygen availability. The increased culture aeration enhances pimaricin production in S. natalensis, however the excess of O(2) consumption can lead to an intracellular ROS imbalance that is harmful to the cell. The adaptive physiological response of S. natalensis upon the addition of exogenous H(2)O(2) suggested that the modulation of the intracellular ROS levels, through the activation of the H(2)O(2) inducible catalase during the late exponential growth phase, can alter the production of pimaricin. With the construction of defective mutants on the H(2)O(2) related enzymes SodF, AhpCD and KatA1, an effective and enduring modulation of intracellular ROS was achieved. Characterization of the knock-out strains revealed different behaviours regarding pimaricin production: whilst the superoxide dismutase defective mutant presented low levels of pimaricin production compared to the wild-type, the mutants defective on the H(2)O(2)-detoxifying enzymes displayed a pimaricin overproducer phenotype. Using physiological and molecular approaches we report a crosstalk between oxidative stress and secondary metabolism regulatory networks. Our results reveal that the redox-based regulation network triggered by an imbalance of the intracellular ROS homeostasis is also able to modulate the biosynthesis of pimaricin in S. natalensis.

PMID:
22114674
[PubMed - indexed for MEDLINE]
PMCID:
PMC3219662
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk