Format

Send to:

Choose Destination
See comment in PubMed Commons below
Opt Express. 2011 Nov 21;19(24):23831-44. doi: 10.1364/OE.19.023831.

In vivo depth-resolved oxygen saturation by Dual-Wavelength Photothermal (DWP) OCT.

Author information

  • 1Department of Ophthalmology, The University of Texas Health Science Center, San Antonio, Texas 78229, USA. rkuranov@yahoo.com

Abstract

Microvasculature hemoglobin oxygen saturation (SaO2) is important in the progression of various pathologies. Non-invasive depth-resolved measurement of SaO2 levels in tissue microvasculature has the potential to provide early biomarkers and a better understanding of the pathophysiological processes allowing improved diagnostics and prediction of disease progression. We report proof-of-concept in vivo depth-resolved measurement of SaO(2) levels in selected 30 µm diameter arterioles in the murine brain using Dual-Wavelength Photothermal (DWP) Optical Coherence Tomography (OCT) with 800 nm and 770 nm photothermal excitation wavelengths. Depth location of back-reflected light from a target arteriole was confirmed using Doppler and speckle contrast OCT images. SaO(2) measured in a murine arteriole with DWP-OCT is linearly correlated (R(2)=0.98) with systemic SaO(2) values recorded by a pulse-oximeter. DWP-OCT are steadily lower (10.1%) than systemic SaO(2) values except during pure oxygen breathing. DWP-OCT is insensitive to OCT intensity variations and is a candidate approach for in vivo depth-resolved quantitative imaging of microvascular SaO(2) levels.

© 2011 Optical Society of America

PMID:
22109408
[PubMed - indexed for MEDLINE]
PMCID:
PMC3482904
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Loading ...
    Write to the Help Desk