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Opt Express. 2011 Nov 21;19(24):23716-26. doi: 10.1364/OE.19.023716.

Parallelized STED fluorescence nanoscopy.

Author information

  • 1German Cancer Research Center (DKFZ), Optical Nanoscopy Division, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.

Abstract

We introduce a parallelized STED microscope featuring m = 4 pairs of scanning excitation and STED beams, providing m-fold increased imaging speed of a given sample area, while maintaining basically all of the advantages of single beam scanning. Requiring only a single laser source and fiber input, the setup is inherently aligned both spatially and temporally. Given enough laser power, the design is readily scalable to higher degrees of parallelization m.

© 2011 Optical Society of America

PMID:
22109398
[PubMed - indexed for MEDLINE]
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