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Proteomics. 2012 Jan;12(2):236-40. doi: 10.1002/pmic.201100166. Epub 2011 Dec 20.

Sites of ubiquitin attachment in Saccharomyces cerevisiae.

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  • 1Howard Hughes Medical Institute, University of Washington, Seattle, WA, USA.

Abstract

Sites of ubiquitin modification have been identified by mass spectrometry based on the increase in molecular mass of a tryptic peptide carrying two additional glycine residues from the ubiquitin moiety. However, such peptides with GG shifts have been difficult to discover. We identify 870 unique sites of ubiquitin attachment on 438 different proteins of the yeast Saccharomyces cerevisiae.

Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PMID:
22106047
[PubMed - indexed for MEDLINE]
PMCID:
PMC3337332
Free PMC Article

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