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    Cancer Lett. 2012 Mar;316(1):105-12. Epub 2011 Oct 29.

    miR-21 functionally interacts with the 3'UTR of chemokine CCL20 and down-regulates CCL20 expression in miR-21 transfected colorectal cancer cells.

    Source

    Department of General, Visceral, Vascular and Paediatric Surgery, University of the Saarland, 66421 Homburg/Saar, Germany.

    Abstract

    As deregulation of miRNAs and chemokine CCL20 was shown to play a role in colorectal cancer (CRC) pathogenesis, we analyzed the functional interactions of candidate miRNAs with CCL20 mRNA. After target prediction software programs indicated a role for miR-21 in CCL20 regulation, we applied the luciferase reporter assay system to demonstrate that miR-21 functionally interacts with the 3'UTR of CCL20 mRNA and down-regulates CCL20 in miR-21 mimic transfected CRC cell lines (Caco-2, SW480 and SW620). Thus, regulation of CCL20 expression by miR-21 might be a regulatory mechanism involved in progression of CRC.

    Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

    PMID:
    22099878
    [PubMed - indexed for MEDLINE]

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