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Biophys J. 2011 Nov 16;101(10):L50-2. doi: 10.1016/j.bpj.2011.10.022. Epub 2011 Nov 15.

An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy.

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  • 1Graduate School of Engineering, Yokohama National University, Yokohama, Japan.


A novel, to our knowledge, in situ photoirradiation system for solid-state NMR measurements is improved and demonstrated to successfully identify the M-photointermediate of pharaonis phoborhodopsin (ppR or sensory rhodopsin II), that of the complex with transducer (ppR/pHtrII), and T204A mutant embedded in a model membrane. The (13)C NMR signals from [20-(13)C]retinal-ppR and ppR/pHtrII revealed that multiple M-intermediates with 13-cis, 15-anti retinal configuration coexisted under the continuously photoirradiated condition. NMR signals observed from the photoactivated retinal provide insights into the process of photocycle in the ppR/pHtrII complex.

Copyright © 2011 Biophysical Society. Published by Elsevier Inc. All rights reserved.

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