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    EMBO J. 1990 Oct;9(10):3179-89.

    The recognition component of the N-end rule pathway.

    Bartel B, Wünning I, Varshavsky A.

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    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

    Abstract

    The N-end rule-based degradation signal, which targets a protein for ubiquitin-dependent proteolysis, comprises a destabilizing amino-terminal residue and a specific internal lysine residue. We report the isolation and functional analysis of a gene (UBR1) for the N-end recognizing protein of the yeast Saccharomyces cerevisiae. UBR1 encodes a approximately 225 kd protein with no significant sequence similarities to other known proteins. Null ubr1 mutants are viable but are unable to degrade the substrates of the N-end rule pathway. These mutants are partially defective in sporulation and grow slightly more slowly than their wild-type counterparts. The UBR1 protein specifically binds in vitro to proteins bearing amino-terminal residues that are destabilizing according to the N-end rule, but does not bind to otherwise identical proteins bearing stabilizing amino-terminal residues.

    PMID:
    2209542
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC552047
    Free PMC Article

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