Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Gen Physiol. 2011 Dec;138(6):641-9. doi: 10.1085/jgp.201110723. Epub 2011 Nov 14.

Expression and characterization of the bacterial mechanosensitive channel MscS in Xenopus laevis oocytes.

Author information

  • 1Department of Biology, Washington University in Saint Louis, Saint Louis, MO 63130, USA.

Abstract

We have successfully expressed and characterized mechanosensitive channel of small conductance (MscS) from Escherichia coli in oocytes of the African clawed frog, Xenopus laevis. MscS expressed in oocytes has the same single-channel conductance and voltage dependence as the channel in its native environment. Two hallmarks of MscS activity, the presence of conducting substates at high potentials and reversible adaptation to a sustained stimulus, are also exhibited by oocyte-expressed MscS. In addition to its ease of use, the oocyte system allows the user to work with relatively large patches, which could be an advantage for the visualization of membrane deformation. Furthermore, MscS can now be compared directly to its eukaryotic homologues or to other mechanosensitive channels that are not easily studied in E. coli.

PMID:
22084416
[PubMed - indexed for MEDLINE]
PMCID:
PMC3226970
Free PMC Article

Images from this publication.See all images (4)Free text

Figure 1.
Figure 2.
Figure 3.
Figure 4.
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk