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    Science. 2011 Nov 11;334(6057):817-21.

    tRNAs marked with CCACCA are targeted for degradation.

    Wilusz JE, Whipple JM, Phizicky EM, Sharp PA.

    Source

    Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. wilusz@mit.edu

    Abstract

    The CCA-adding enzyme [ATP(CTP):tRNA nucleotidyltransferase] adds CCA to the 3' ends of transfer RNAs (tRNAs), a critical step in tRNA biogenesis that generates the amino acid attachment site. We found that the CCA-adding enzyme plays a key role in tRNA quality control by selectively marking structurally unstable tRNAs and tRNA-like small RNAs for degradation. Instead of adding CCA to the 3' ends of these transcripts, CCA-adding enzymes from all three kingdoms of life add CCACCA. In addition, hypomodified mature tRNAs are subjected to CCACCA addition as part of a rapid tRNA decay pathway in vivo. We conjecture that CCACCA addition is a universal mechanism for controlling tRNA levels and preventing errors in translation.

    PMID:
    22076379
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC3273417
    Free PMC Article

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