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Biomed Opt Express. 2011 Nov 1;2(11):3021-36. doi: 10.1364/BOE.2.003021. Epub 2011 Oct 5.

Imaging workflow and calibration for CT-guided time-domain fluorescence tomography.

Abstract

In this study, several key optimization steps are outlined for a non-contact, time-correlated single photon counting small animal optical tomography system, using simultaneous collection of both fluorescence and transmittance data. The system is presented for time-domain image reconstruction in vivo, illustrating the sensitivity from single photon counting and the calibration steps needed to accurately process the data. In particular, laser time- and amplitude-referencing, detector and filter calibrations, and collection of a suitable instrument response function are all presented in the context of time-domain fluorescence tomography and a fully automated workflow is described. Preliminary phantom time-domain reconstructed images demonstrate the fidelity of the workflow for fluorescence tomography based on signal from multiple time gates.

KEYWORDS:

(170.0110) Imaging systems, (170.3010) Image reconstruction techniques, (170.3660) Light propagation in tissues

PMID:
22076264
[PubMed]
PMCID:
PMC3207372
Free PMC Article

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