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Toxins (Basel). 2010 Jun;2(6):1265-78. doi: 10.3390/toxins2061265. Epub 2010 Jun 1.

Alpha-tocopherol counteracts the cytotoxicity induced by ochratoxin a in primary porcine fibroblasts.

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  • 1Università degli Studi di Milano, Department of Veterinary Science and Technology for Food Safety, Via Trentacoste 2, I-20134 Milan, Italy. eleonora.fusi@unimi.it

Abstract

The aims of the current study were to determine the half-lethal concentration of ochratoxin A (OTA) as well as the levels of lactate dehydrogenase release and DNA fragmentation induced by OTA in primary porcine fibroblasts, and to examine the role of α-tocopherol in counteracting its toxicity. Cells showed a dose-, time- and origin-dependent (ear vs. embryo) sensitivity to ochratoxin A. Pre-incubation for 3 h with 1 nM α-tocopherol significantly (P < 0.01) reduced OTA cytotoxicity, lactate dehydrogenase release and DNA damage in both fibroblast cultures. These findings indicate that α-tocopherol supplementation may counteract short-term OTA toxicity, supporting its defensive role in the cell membrane.

KEYWORDS:

DNA damage; fibroblasts; ochratoxin A; swine; α-tocopherol

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