Microbacterium suwonense sp. nov., isolated from cow dung

J Microbiol. 2011 Oct;49(5):852-6. doi: 10.1007/s12275-011-1036-y. Epub 2011 Nov 9.

Abstract

An actinomycete strain, designated M1T8B9(T), was isolated from cow dung in Suwon, Republic of Korea. The isolate was a Gram-positive, nonmotile, and non-spore-forming bacterium. Phylogenetic evaluation based on 16S rRNA gene sequence similarity showed that this isolate belongs to the genus Microbacterium, with its closest neighbors being Microbacterium soli DCY17(T) (98.2%) and Microbacterium esteraromaticum DSM 8609(T) (98.0%). The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, and one unknown glycolipid. Strain M1T8B9(T) contained the major fatty acids C(15:0) anteiso, C(16:0) iso, C (17:0) anteiso, and C(15:0) iso, and the cell-wall peptidoglycan was of type B2β. According to DNA-DNA hybridization studies, strain M1T8B9(T) showed 42% and 26% relatedness with M. soli DCY17(T) and M. esteraromaticum DSM 8609(T), respectively. On the basis of the data presented, strain M1T8B9(T) is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium suwonense sp. nov. is proposed. The type strain is M1T8B9(T) (=KACC 14058(T) =NBRC 106310(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / chemistry
  • Actinomycetales / classification*
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification*
  • Animals
  • Cattle
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Feces / microbiology*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Peptidoglycan / analysis
  • Phospholipids / analysis
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Peptidoglycan
  • Phospholipids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/GQ246683