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FEMS Immunol Med Microbiol. 2012 Feb;64(1):111-4. doi: 10.1111/j.1574-695X.2011.00882.x.

Tryptose phosphate broth improves Rickettsia felis replication in mammalian cells.

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  • 1URMITE UMR CNRS 6236-IRD198, Faculté de Médecine et de Pharmacie, Université de la Méditerranée, Marseille, France.

Abstract

In cell culture, Rickettsia felis grows only at low temperatures (< 31 °C). Therefore, its ability to enter, survive and grow in cell lines has primarily been tested in cells derived from amphibians and arthropods, which naturally grow at low temperatures, and only infrequently in mammalian cells. We subcultured R. felis in mammalian cells for more than 10 passages using media supplemented with tryptose phosphate broth (TPB) and found that TPB is critical for optimal growth of R. felis in mammalian cells.

PMID:
22066776
[PubMed - indexed for MEDLINE]
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