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J Cell Biol. 2011 Nov 14;195(4):657-71. doi: 10.1083/jcb.201104045. Epub 2011 Nov 7.

Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin.

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  • 1Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA.


Yeast is a powerful model organism for dissecting the temporal stages and choreography of the complex protein machinery during endocytosis. The only known mechanism for endocytosis in yeast is clathrin-mediated endocytosis, even though clathrin-independent endocytic pathways have been described in other eukaryotes. Here, we provide evidence for a clathrin-independent endocytic pathway in yeast. In cells lacking the clathrin-binding adaptor proteins Ent1, Ent2, Yap1801, and Yap1802, we identify a second endocytic pathway that depends on the GTPase Rho1, the downstream formin Bni1, and the Bni1 cofactors Bud6 and Spa2. This second pathway does not require components of the better-studied endocytic pathway, including clathrin and Arp2/3 complex activators. Thus, our results reveal the existence of a second pathway for endocytosis in yeast, which suggests similarities with the RhoA-dependent endocytic pathways of mammalian cells.

© 2011 Prosser et al.

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