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Biosci Biotechnol Biochem. 2011;75(11):2234-6. Epub 2011 Nov 7.

TPS1 terminator increases mRNA and protein yield in a Saccharomyces cerevisiae expression system.

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  • 1TOYOTA Central R&D Labs, Inc, Nagakute, Aichi, Japan.

Abstract

Both terminators and promoters regulate gene expression. In Saccharomyces cerevisiae, the TPS1 terminator (TPS1t), coupled to a gene encoding a fluorescent protein, produced more transgenic mRNA and protein than did similar constructs containing other terminators, such as CYC1t, TDH3t, and PGK1t. This suggests that TPS1t can be used as a general terminator in the development of metabolically engineered yeast in high-yield systems.

PMID:
22056446
[PubMed - indexed for MEDLINE]
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