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Mol Cell Proteomics. 2012 Mar;11(3):M111.013458. doi: 10.1074/mcp.M111.013458. Epub 2011 Oct 31.

Antibody-based protein profiling of the human chromosome 21.

Author information

  • 1Science for Life Laboratory, Royal Institute of Technology, SE-17121 Solna, Sweden. mathias.uhlen@scilifelab.se

Abstract

The Human Proteome Project has been proposed to create a knowledge-based resource based on a systematical mapping of all human proteins, chromosome by chromosome, in a gene-centric manner. With this background, we here describe the systematic analysis of chromosome 21 using an antibody-based approach for protein profiling using both confocal microscopy and immunohistochemistry, complemented with transcript profiling using next generation sequencing data. We also describe a new approach for protein isoform analysis using a combination of antibody-based probing and isoelectric focusing. The analysis has identified several genes on chromosome 21 with no previous evidence on the protein level, and the isoform analysis indicates that a large fraction of human proteins have multiple isoforms. A chromosome-wide matrix is presented with status for all chromosome 21 genes regarding subcellular localization, tissue distribution, and molecular characterization of the corresponding proteins. The path to generate a chromosome-specific resource, including integrated data from complementary assay platforms, such as mass spectrometry and gene tagging analysis, is discussed.

PMID:
22042635
[PubMed - indexed for MEDLINE]
PMCID:
PMC3316724
Free PMC Article

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