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Nat Methods. 2011 Oct 30;8(12):1053-5. doi: 10.1038/nmeth.1750.

Enhanced Y1H assays for Arabidopsis.

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  • 1Department of Plant Biology and Genome Center, University of California Davis, Davis, California, USA.

Abstract

We present an Arabidopsis thaliana full-length transcription factor resource of 92% of root stele-expressed transcription factors and 74.5% of root-expressed transcription factors. We demonstrate its use with enhanced yeast one-hybrid (eY1H) screening for rapid, systematic mapping of plant transcription factor-promoter interactions. We identified 158 interactions with 13 stele-expressed promoters, many of which occur physically or are regulatory in planta.

PMID:
22037706
[PubMed - indexed for MEDLINE]
PMCID:
PMC3821074
Free PMC Article

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