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Cell Cycle. 2011 Oct 15;10(20):3527-32. doi: 10.4161/cc.10.20.17792. Epub 2011 Oct 15.

Generation of a set of conditional analog-sensitive alleles of essential protein kinases in the fission yeast Schizosaccharomyces pombe.

Author information

  • 1Max F Perutz Laboratories, University of Vienna, Vienna, Austria.

Abstract

The genome of the fission yeast Schizosaccharomyces pombe encodes for 17 protein kinases that are essential for viability. Studies of the essential kinases often require the use of mutant strains carrying conditional alleles. To inactivate these kinases conditionally, we applied a recently developed chemical genetic strategy. The mutation of a single residue in the ATP-binding pocket confers sensitivity to small-molecule inhibitors, allowing for specific inactivation of the modified kinase. Using this approach, we constructed conditional analog-sensitive alleles of 13 essential protein kinases in the fission yeast S. pombe.

PMID:
22030861
[PubMed - indexed for MEDLINE]
PMCID:
PMC3266181
Free PMC Article

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