Format

Send to:

Choose Destination
See comment in PubMed Commons below
Semin Cell Dev Biol. 2012 Apr;23(2):192-9. doi: 10.1016/j.semcdb.2011.10.010. Epub 2011 Oct 19.

Next generation sequencing in epigenetics: insights and challenges.

Author information

  • 1Birkbeck University of London, Department of Psychological Sciences, Malet Street, London WC1E 7HX, United Kingdom.

Abstract

The epigenetics community was an early adopter of next generation sequencing (NGS). NGS-based studies have provided detailed and comprehensive views of epigenetic modifications for the genomes of many species and cell types. Recently, DNA methylation has attracted much attention due to the discovery of 5-hydroxymethyl-cytosine and its role in epigenetic reprogramming and pluripotency. This renewed interest has been concomitant with methodological progress enabling, for example, high coverage and single base resolution profiling of the mammalian methylome in small numbers of cells. We summarise this progress and highlight resulting key findings about the complexity of eukaryotic DNA methylation, its role in metazoan genome evolution, epigenetic reprogramming, and its close ties with histone modifications in the context of transcription. Finally, we discuss how fundamental insights gained by NGS, particularly the discovery of widespread allele-specific epigenetic variation in the human genome, have the potential to significantly contribute to the understanding of human common complex diseases.

Copyright © 2011 Elsevier Ltd. All rights reserved.

PMID:
22027613
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk