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Biomed Opt Express. 2011 Oct 1;2(10):2897-904. doi: 10.1364/BOE.2.002897. Epub 2011 Sep 29.

In vivo and in situ cellular imaging full-field optical coherence tomography with a rigid endoscopic probe.

Abstract

Full-field OCT has proved to be a powerful high-resolution cellular imaging tool for biological tissues. However the standard bulk full-field OCT setup does not match the size requirements for most in situ and in vivo imaging applications. We adapted its principle into a rigid needle-like probe using two coupled interferometers and incoherent illumination: an external processing interferometer is used for in-depth scanning, while a distal common-path interferometer at the tip of the probe collects light backscattered from the tissue. Our experimental setup achieves an axial and transversal resolution in tissue of 1.8 µm and 3.5 µm respectively, for a sensitivity of -80 dB. We present ex vivo images of human breast tissue, and in vivo images of different areas of human skin, which reveal cellular-level structures.

KEYWORDS:

(110.2760) Gradient-index lenses, (110.4500) Optical coherence tomography, (170.2150) Endoscopic imaging, (170.3880) Medical and biological imaging, (180.3170) Interference microscopy

PMID:
22025991
[PubMed]
PMCID:
PMC3191453
Free PMC Article

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