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J Urol. 2011 Dec;186(6):2448-54. doi: 10.1016/j.juro.2011.08.010. Epub 2011 Oct 21.

Amelioration of renal ischemia-reperfusion injury with a novel protective cocktail.

Author information

  • 1Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA. thambi_dorai@nymc.edu

Abstract

PURPOSE:

Extended warm ischemia during partial nephrectomy can lead to considerable renal injury. Using a rat model of renal ischemia we examined the ability of a unique renoprotective cocktail to ameliorate warm ischemia-reperfusion injury.

MATERIALS AND METHODS:

A warm renal ischemia model was developed using 60 Sprague-Dawley® rats. The left renal artery was clamped for 40 minutes, followed by 48 hours of reperfusion. A renoprotective cocktail of a mixture of specific growth factors, mitochondria protecting biochemicals and Manganese-Porphyrin (MnTnHex-2-PyP(5+)) was given intramuscularly at -24, 0 and 24 hours after surgery. At 48 hours the 2 kidneys were harvested and examined with hematoxylin and eosin, and periodic acid-Schiff stains. Protein and gene expression were also analyzed to determine ischemia markers and the antioxidant response.

RESULTS:

Compared to ischemic controls, kidneys treated with the renoprotective cocktail showed significant reversal of morphological changes and a significant decrease in the specific ischemic markers lipocalin-2, mucin-1 and galectin-3. Quantitative reverse transcriptase-polymerase chain reaction revealed up-regulation of several antioxidant genes in treated animals.

CONCLUSIONS:

According to histopathological and several molecular measures our unique renoprotective cocktail mitigated ischemia-reperfusion injury.

Copyright © 2011 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

PMID:
22019164
[PubMed - indexed for MEDLINE]
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