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J Urol. 2011 Dec;186(6):2448-54. doi: 10.1016/j.juro.2011.08.010. Epub 2011 Oct 21.

Amelioration of renal ischemia-reperfusion injury with a novel protective cocktail.

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  • 1Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA. thambi_dorai@nymc.edu



Extended warm ischemia during partial nephrectomy can lead to considerable renal injury. Using a rat model of renal ischemia we examined the ability of a unique renoprotective cocktail to ameliorate warm ischemia-reperfusion injury.


A warm renal ischemia model was developed using 60 Sprague-Dawley® rats. The left renal artery was clamped for 40 minutes, followed by 48 hours of reperfusion. A renoprotective cocktail of a mixture of specific growth factors, mitochondria protecting biochemicals and Manganese-Porphyrin (MnTnHex-2-PyP(5+)) was given intramuscularly at -24, 0 and 24 hours after surgery. At 48 hours the 2 kidneys were harvested and examined with hematoxylin and eosin, and periodic acid-Schiff stains. Protein and gene expression were also analyzed to determine ischemia markers and the antioxidant response.


Compared to ischemic controls, kidneys treated with the renoprotective cocktail showed significant reversal of morphological changes and a significant decrease in the specific ischemic markers lipocalin-2, mucin-1 and galectin-3. Quantitative reverse transcriptase-polymerase chain reaction revealed up-regulation of several antioxidant genes in treated animals.


According to histopathological and several molecular measures our unique renoprotective cocktail mitigated ischemia-reperfusion injury.

Copyright © 2011 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

[PubMed - indexed for MEDLINE]
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