Aerobic remediation of petroleum sludge through soil supplementation: microbial community analysis

J Hazard Mater. 2011 Dec 15:197:80-7. doi: 10.1016/j.jhazmat.2011.09.061. Epub 2011 Sep 21.

Abstract

The effect of soil concentration on the aerobic degradation of real-field petroleum sludge was studied in slurry phase reactor. Total petroleum hydrocarbons (TPH) and polycyclic aromatic hydrocarbons (PAHs) showed effective removal but found to depend on the soil concentration. Aromatic fraction (48.12%) documented effective degradation compared to aliphatics (47.31%), NSO (28.69%) and asphaltenes (26.66%). PAHs profile showed efficient degradation of twelve individual aromatic compounds where lower ring compounds showed relatively higher degradation efficiency compared to the higher ring compounds. The redox behaviour and dehydrogenase activity showed a linear increment with the degradation pattern. Microbial community composition and changes during bioremediation were studied using denaturing gradient gel electrophoresis (DGGE). Among the 12 organisms identified, Proteobacteria was found to be dominant representing 50% of the total population (25% of γ-proteobacteria; 16.6% of β-proteobacteria; 8.3% of α-proteobacteria), while 33.3% were of uncultured bacteria and 16.6% were of firmicutes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerobiosis
  • Base Sequence
  • Biodegradation, Environmental*
  • DNA Primers
  • Environmental Restoration and Remediation / methods*
  • Petroleum / metabolism*
  • Phylogeny
  • Polycyclic Compounds / metabolism
  • Polymerase Chain Reaction
  • Proteobacteria / classification
  • Proteobacteria / enzymology
  • Proteobacteria / metabolism*
  • Sewage*
  • Soil Microbiology*
  • Soil Pollutants / metabolism*

Substances

  • DNA Primers
  • Petroleum
  • Polycyclic Compounds
  • Sewage
  • Soil Pollutants