J Clin Microbiol. 2011 Dec;49(12):4352-5. doi: 10.1128/JCM.06092-11. Epub 2011 Oct 19.

True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis.

Rudkjøbing VB, Thomsen TR, Alhede M, Kragh KN, Nielsen PH, Johansen UR, Givskov M, Høiby N, Bjarnsholt T.

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Department of Biotechnology, Chemistry, and Environmental Engineering, Faculty of Engineering and Science, Aalborg University, Aalborg, Denmark.

Abstract

Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens.

PMID:: 22012018; [PubMed - indexed for MEDLINE]
PMCID:: PMC3233003

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GENBANK/JN802672
GENBANK/JN802673
GENBANK/JN802674
GENBANK/JN802675
GENBANK/JN802676
GENBANK/JN802677
GENBANK/JN802678
GENBANK/JN802679
GENBANK/JN802680
GENBANK/JN802681
GENBANK/JN802682
GENBANK/JN802683
GENBANK/JN802684
GENBANK/JN802685
GENBANK/JN802686
GENBANK/JN802687
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GENBANK/JN802689
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GENBANK/JN802691
GENBANK/JN802692
GENBANK/JN802693
GENBANK/JN802694
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GENBANK/JN802696
GENBANK/JN802697
GENBANK/JN802698
GENBANK/JN802699
GENBANK/JN802700
GENBANK/JN802701
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