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Proteomics. 2011 Dec;11(24):4610-21. doi: 10.1002/pmic.201100135. Epub 2011 Nov 23.

Preventing and troubleshooting artefacts in saturation labelled fluorescence 2-D difference gel electrophoresis (saturation DiGE).

Author information

  • 1Centre for Cell Engineering, Division of Biochemistry and Cell Biology, Joseph Black Building, University of Glasgow, Glasgow, UK. laura.mcnamara@glasgow.ac.uk

Abstract

Saturation DiGE is a powerful but challenging technique for the characterisation of changes in protein expression between two or more scarce samples. In this paper, measures to prevent and troubleshoot artefacts in the saturation DiGE workflow are discussed, with illustration of some examples as they may be encountered in gel images or analysis.

Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PMID:
22002958
[PubMed - indexed for MEDLINE]
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