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Nat Chem Biol. 2011 Oct 16;7(12):885-7. doi: 10.1038/nchembio.687.

N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO.

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  • 1Department of Chemistry, University of Chicago, Chicago, Illinois, USA.

Erratum in

  • Nat Chem Biol. 2012 Dec;8(12):1008.

Abstract

We report here that fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m(6)A) residues in RNA in vitro. FTO knockdown with siRNA led to increased amounts of m(6)A in mRNA, whereas overexpression of FTO resulted in decreased amounts of m(6)A in human cells. We further show the partial colocalization of FTO with nuclear speckles, which supports the notion that m(6)A in nuclear RNA is a major physiological substrate of FTO.

PMID:
22002720
[PubMed - indexed for MEDLINE]
PMCID:
PMC3218240
Free PMC Article

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