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Methods Mol Biol. 2012;799:405-19. doi: 10.1007/978-1-61779-346-2_22.

A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens.

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  • 1Division of Cancer Sciences, Genetic Vaccines Group, University of Southampton Medical School, Southampton, UK.


DNA-based vaccines are currently being developed for treating a diversity of human diseases including cancers, autoimmune conditions, allergies, and microbial infections. In this chapter, we present a general protocol that can be used as a starting point for developing DNA vaccines to pathogen-derived antigens, using Neisseria meningitidis as an example. In addition, we describe a fusion gene-based vaccine protocol for increasing the potency of DNA vaccines that are based on poorly immunogenic antigens such as short pathogen-derived polypeptides. Finally, we provide a safe and effective protocol for delivery of DNA vaccines, based on intramuscular injection followed by electroporation.

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