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Biomed Opt Express. 2011 Sep 1;2(9):2590-9. doi: 10.1364/BOE.2.002590. Epub 2011 Aug 15.

Holographic UV laser microsurgery.

Abstract

We use a spatial light modulator (SLM) to diffract a single UV laser pulse to ablate multiple points on a Drosophila embryo. This system dynamically generates a phase hologram for ablating a user-defined pattern fast enough to be used with living, and thus moving, tissue. We demonstrate the ability of this single-pulse multi-point system to perform two experiments that are very difficult for conventional microsurgery-isolating single cells in vivo and measuring fast retractions from large incisions.

KEYWORDS:

(070.6120) Spatial light modulators, (090.1995) Digital holography, (170.1020) Ablation of tissue

PMID:
21991551
[PubMed]
PMCID:
PMC3184868
Free PMC Article

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