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J Plant Physiol. 2012 Jan 15;169(2):193-205. doi: 10.1016/j.jplph.2011.09.002. Epub 2011 Oct 5.

Transcriptome profiling characterizes phosphate deficiency effects on carbohydrate metabolism in rice leaves.

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  • 1Institute of Agricultural Science & Technology, Chonbuk National University, Jeonju, Jeollabuk-do 561-756, Republic of Korea.

Abstract

Phosphorus (P) is a structural component of nucleic acids and phospholipids and plays important roles in plant growth and development. P accumulation was significantly reduced (about 35%) in rice leaves from plants grown under low (32 μM) P compared to 320 μM P grown plants. Genome response to low P was examined using the rice 60K oligonucleotide DNA microarrays. At the threshold significance of |log₂| fold>2.0, 21,033 genes (about 33.7% of all genes on the microarray) were affected by P deficiency. Among all genes on the microarray, 4271 genes were sorted into 51 metabolic pathways. Low P affected 1494 (35.0%) genes and the largest category of genes was related to sucrose degradation to ethanol and lactate pathway. To survey the role of P in rice, 25 pathways were selected based on number of affected genes. Among these pathways, cytosolic glycolysis contained the least number of upregulated but most down-regulated genes. Low P decreased glucose, pyruvate and chlorophyll, and genes related to carbon metabolism and chlorophyllide a biosynthesis. However, sucrose and starch levels increased. These results indicate that P nutrition affects diverse metabolic pathways mostly related to glucose, pyruvate, sucrose, starch, and chlorophyll a.

Copyright © 2011 Elsevier GmbH. All rights reserved.

PMID:
21978493
[PubMed - indexed for MEDLINE]
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