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J Clin Microbiol. 2011 Dec;49(12):4356-60. doi: 10.1128/JCM.05167-11. Epub 2011 Oct 5.

Dried culture spots for Xpert MTB/RIF external quality assessment: results of a phase 1 pilot study in South Africa.

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  • 1Department of Molecular Medicine and Haematology, University of the Witwatersrand, 7 York Road, Parktown, Johannesburg, South Africa. lesley.scott@nhls.ac.za


Implementation of Xpert MTB/RIF requires quality assessment. A pilot program using dried culture spots (DCSs) of inactivated Mycobacterium tuberculosis is described. Of 274 DCS results received, 2.19% generated errors; the remainder yielded 100% correct Mycobacterium tuberculosis detection. The probe A cycle threshold (C(T)) variability of three DCS batches was ≤ 3.47. The study of longer-term DCS stability is ongoing.

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