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ACS Chem Biol. 2011 Dec 16;6(12):1314-20. doi: 10.1021/cb200155t. Epub 2011 Oct 13.

A map of minor groove shape and electrostatic potential from hydroxyl radical cleavage patterns of DNA.


DNA shape variation and the associated variation in minor groove electrostatic potential are widely exploited by proteins for DNA recognition. Here we show that the hydroxyl radical cleavage pattern is a quantitative measure of DNA backbone solvent accessibility, minor groove width, and minor groove electrostatic potential, at single nucleotide resolution. We introduce maps of DNA shape and electrostatic potential as tools for understanding how proteins recognize binding sites in a genome. These maps reveal periodic structural signals in yeast and Drosophila genomic DNA sequences that are associated with positioned nucleosomes.

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