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Lipids Health Dis. 2011 Sep 30;10:173. doi: 10.1186/1476-511X-10-173.

MicroRNA profile of polyunsaturated fatty acid treated glioma cells reveal apoptosis-specific expression changes.

Author information

  • 1Functional Genomics Laboratory, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary. nora@avidinbiotech.com

Abstract

BACKGROUND:

Polyunsaturated fatty acids (PUFAs) such as γ-linolenic acid (GLA), arachidonic acid (AA) and docosahexaenoic acid (DHA) have cytotoxic action on glioma cells.

RESULTS:

We evaluated the cytotoxic action of GLA, AA and DHA on glioma cells with specific reference to the expression of miRNAs. Relative expression of miRNAs were assessed by using high throughput nanocapillary real-time PCR. Most of the miRNA target genes that showed altered expression could be classified as apoptotic genes and were up-regulated by PUFA or temozolomide treatment, while similar treatments resulted in repression of the corresponding mRNAs, such as cox2, irs1, irs2, ccnd1, itgb3, bcl2, sirt1, tp53inp1 and k-ras.

CONCLUSIONS:

Our results highlight involvement of miRNAs in the induction of apoptosis in glioma cells by fatty acids and temozolomide.

PMID:
21961478
[PubMed - indexed for MEDLINE]
PMCID:
PMC3203338
Free PMC Article

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