Transcriptome analysis revealing gene expression patterns by E6 oncoproteins. A. Hierarchical cluster analysis of the wild-type E6 or E6 D25E viral oncogene expression microarrays. A cluster-based representation of altered genes in E6 wild-type or E6 D25E expressed C33A with intensity provided for each cell line, normalized to the control (C33A cells not expressing E6). Genes that were up-regulated relative to control are shown in red and those that were down-regulated are green. The expression levels of these genes were altered ≥1.5-fold or ≤0.6-fold in E6-expressing cell lines compared with controls (p < 0.05). Gene symbols are shown for all in the right row. The hybridization was conducted in duplicate and denoted with 1 and 2. B. A venn diagram showing the numbers of genes regulated by wild-type E6 or E6 D25E. The numbers of up- or down-regulated genes that responded commonly or uniquely in response to the expression of each E6 protein are shown in red or blue arrows, respectively. The complete list of 211 genes is shown in additional file 1 and the entire microarray data set has been deposited in the Gene Ontology Consortium database (http://www.geneontology.org/index.shtml). C. Gene ontology-based analysis.

Confirmation of HPV-16 E6 oncogenes expression and effects on the transcription of endogenous survivin gene in C33A cell lines with e6 oncogenes. A. Quantification of mRNA of wild-type E6 and E6 D25E in C33A cells by RT-PCR performed using OneStep RT-PCR kits. GAPDH mRNA was measured as a loading control. B. Protein expression analysis of wild-type E6 or E6 D25E using confocal microscopy. Caski cells were used as a positive control. C. Quantative real-time PCR specific for survivin gene.