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Cancer Res. 2011 Nov 1;71(21):6708-17. doi: 10.1158/0008-5472.CAN-11-1472. Epub 2011 Sep 21.

RNA helicase DDX5 is a p53-independent target of ARF that participates in ribosome biogenesis.

Author information

  • 1BRIGHT Institute and Department of Internal Medicine, Siteman Cancer Center, Washington University School of Medicine, St. Louis, Missouri, USA.

Abstract

The p19ARF tumor suppressor limits ribosome biogenesis and responds to hyperproliferative signals to activate the p53 checkpoint response. Although its activation of p53 has been well characterized, the role of ARF in restraining nucleolar ribosome production is poorly understood. Here we report the use of a mass spectroscopic analysis to identify protein changes within the nucleoli of Arf-deficient mouse cells. Through this approach, we discovered that ARF limited the nucleolar localization of the RNA helicase DDX5, which promotes the synthesis and maturation of rRNA, ultimately increasing ribosome output and proliferation. ARF inhibited the interaction between DDX5 and nucleophosmin (NPM), preventing association of DDX5 with the rDNA promoter and nuclear pre-ribosomes. In addition, Arf-deficient cells transformed by oncogenic RasV12 were addicted to DDX5, because reduction of DDX5 was sufficient to impair RasV12-driven colony formation in soft agar and tumor growth in mice. Taken together, our findings indicate that DDX5 is a key p53-independent target of the ARF tumor suppressor and is a novel non-oncogene participant in ribosome biogenesis.

©2011 AACR.

PMID:
21937682
[PubMed - indexed for MEDLINE]
PMCID:
PMC3206203
Free PMC Article

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