Morphological characteristics of Zaire ebolavirus (ZEBOV) replication. (A) Ultrathin section of a ZEBOV-infected Vero cell containing large viral inclusions. The inclusions are composed of granular material (1; also shown in (C)) and rod-like nucleocapsids. Released virions are indicated by long arrows and are shown in the inserts. (B) Cross section of viral inclusion containing nucleocapsids. (C) Longitudinal section of viral inclusion containing nucleocapsids. (D–F) Budding of viral particles. In the initial step of budding a particle can be positioned parallel (D and E, cross and longitudinal sections), or perpendicular (F) to the membrane and then subsequently is enveloped. Short arrows indicate the cellular plasma membrane. 2—nucleus, 3—nucleolus, 4—Golgi zone. Bars in Figures 2B–F correspond to 250 nm. Thick part of frame around cross-sectioned virion corresponds to 120 nm, and thick part of frame around longitudinal section corresponds to 160 nm. Transmission electron microscopy. Cells were fixed at 16 h p.i.

Transmission electron microscopy of ultrathin sections of lymph node tissue from filovirus-infected African green monkeys infected with ZEBOV (A,C–E) or MARV (B). Tissues were fixed at 4 days p.i. (A) Small lymphocyte showing normal nuclear morphology with large areas of heterochromatin. (B,C) Apoptotic lymphocytes. Typical signs of apoptosis such as chromatin condensation and marginal location of chromatin are visible. (D,E) Apoptotic lymphocytes being engulfed by macrophages. (D) shows initial stages of phagocytosis. The lymphocyte is engulfed by a macrophage. The macrophage shown in (E) contains several destroyed apoptotic lymphocytes. Arrows show filoviral particles. 1—highly condensed heterochromatin; 2—monocyte showing normal nuclear morphology; 3—nucleus of macrophage. Bars correspond to 2 μm.

Scheme of the filovirus infection cycle. Filoviruses enter the cell by receptor-mediated endocytosis or macropinocytosis. After fusion of the viral and cellular membrane, the nucleocapsid is released into the cytoplasm and serves as a template for transcription and replication. The replicated RNA is encapsidated by the nucleocapsid proteins. The newly synthesized nucleocapsids are transported to the sites of viral release, where budding takes place.

Transmission electron microscopy of ultrathin sections of tissues from animals experimentally infected with filoviruses. Tissues were fixed at day 5 or 6 p.i. (A) Ultrathin section of liver tissue from ZEBOV-infected rhesus monkey showing an infected macrophage. (B,C) Ultrathin sections of liver tissue from Marburg virus (MARV)-infected guinea pig. Shown are an infected macrophage (B) and an infected hepatocyte (C). (D) Ultrathin section of spleen tissue from ZEBOV-infected African green monkey showing an infected endothelial cell. (E) Ultrathin section of lymphatic node tissue from ZEBOV-infected African green monkey showing a necrotic infected macrophage. (F) Ultrathin section of a necrotic ZEBOV-infected Vero cell showing an infected macrophage. Arrows show vacuolization of endoplasmic reticulum cisterns in cells undergoing non-apoptotic cell death. 1—filovirus inclusions; 2—nucleus; 3—erythrocyte. Bars correspond to 2 μm.