Circular representation of the F. branchiophilum FL-15 genome. Circles represent the following (from the inside out): 1, GC skew [(G − C)/(G + C) using a 2-kbp sliding window] (purple, positive GC skew; orange, negative GC skew); 2, G+C deviation (difference between the average G+C content in a 2-kbp window and the genomic average G+C), where red areas indicate that the deviation is greater than 2 standard deviations; 3, location of ISs; 4, location of tRNA genes (black) and rRNA operons (red); 5, genes with orthologs in F. johnsoniae (blue) or F. psychrophilum (red); 6, core Flavobacterium genome, i.e., genes with orthologs in F. johnsoniae UW101T and F. psychrophilum JIP02/86 (gray); 7, putative horizontal gene transfer regions detected by Alien Hunter (58), where the height corresponds to the score prediction (the higher the score, the more significant the detected region); and 8, regions specific to F. branchiophilum FL-15 (blue). In the outermost circle, a specific region corresponds to at least 10 consecutive noncore genes and has less than 40% of the genes present in F. johnsoniae UW101T or F. psychrophilum JIP02/86. Characteristics of each region (defined by number) are indicated in Table S2 in the supplemental material.