Mol Cell. 2011 Oct 7;44(1):17-28. Epub 2011 Sep 15.

Directional DNA methylation changes and complex intermediate states accompany lineage specificity in the adult hematopoietic compartment.

Hodges E, Molaro A, Dos Santos CO, Thekkat P, Song Q, Uren PJ, Park J, Butler J, Rafii S, McCombie WR, Smith AD, Hannon GJ.

Source

Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.

Abstract

DNA methylation has been implicated as an epigenetic component of mechanisms that stabilize cell-fate decisions. Here, we have characterized the methylomes of human female hematopoietic stem/progenitor cells (HSPCs) and mature cells from the myeloid and lymphoid lineages. Hypomethylated regions (HMRs) associated with lineage-specific genes were often methylated in the opposing lineage. In HSPCs, these sites tended to show intermediate, complex patterns that resolve to uniformity upon differentiation, by increased or decreased methylation. Promoter HMRs shared across diverse cell types typically display a constitutive core that expands and contracts in a lineage-specific manner to fine-tune the expression of associated genes. Many newly identified intergenic HMRs, both constitutive and lineage specific, were enriched for factor binding sites with an implied role in genome organization and regulation of gene expression, respectively. Overall, our studies represent an important reference data set and provide insights into directional changes in DNA methylation as cells adopt terminal fates.

PMID:: 21924933; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Secondary Source ID

Publication Types

MeSH Terms

Secondary Source ID

GEO/GSE31971

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Medical

Stem Cells - MedlinePlus Health Information

Supplemental Content

Save items

Related citations in PubMed

Comprehensive methylome map of lineage commitment from haematopoietic progenitors. [Nature. 2010]
Comprehensive methylome map of lineage commitment from haematopoietic progenitors.
Ji H, Ehrlich LI, Seita J, Murakami P, Doi A, Lindau P, Lee H, Aryee MJ, Irizarry RA, Kim K, et al. Nature. 2010 Sep 16; 467(7313):338-42. Epub 2010 Aug 15.
Final Report on Carcinogens Background Document for Formaldehyde. [Rep Carcinog Backgr Doc. 2010]
Final Report on Carcinogens Background Document for Formaldehyde.
National Toxicology Program. Rep Carcinog Backgr Doc. 2010 Jan; (10-5981):i-512.
Genome-wide promoter DNA methylation dynamics of human hematopoietic progenitor cells during differentiation and aging. [Blood. 2011]
Genome-wide promoter DNA methylation dynamics of human hematopoietic progenitor cells during differentiation and aging.
Bocker MT, Hellwig I, Breiling A, Eckstein V, Ho AD, Lyko F. Blood. 2011 May 12; 117(19):e182-9. Epub 2011 Mar 22.
Review Epigenetic plasticity of chromatin in embryonic and hematopoietic stem/progenitor cells: therapeutic potential of cell reprogramming. [Leukemia. 2008]
Review Epigenetic plasticity of chromatin in embryonic and hematopoietic stem/progenitor cells: therapeutic potential of cell reprogramming.
Zardo G, Cimino G, Nervi C. Leukemia. 2008 Aug; 22(8):1503-18. Epub 2008 Jun 12.
Review Genetics and epigenetics: stability and plasticity during cellular differentiation. [Trends Genet. 2009]
Review Genetics and epigenetics: stability and plasticity during cellular differentiation.
Mohn F, Schübeler D. Trends Genet. 2009 Mar; 25(3):129-36. Epub 2009 Jan 29.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
GEO DataSets
Gene expression and molecular abundance data reported in the current articles that are also included in the curated Gene Expression Omnibus (GEO) DataSets.
Related Project
Related Projects
SRA
Massively-parallel sequencing project data in the Short Read Archive (SRA) that are reported in the current articles.

Recent activity

Clear Turn Off Turn On

Directional DNA methylation changes and complex intermediate states accompany li...
Directional DNA methylation changes and complex intermediate states accompany lineage specificity in the adult hematopoietic compartment.
Mol Cell. 2011 Oct 7 ;44(1):17-28. Epub 2011 Sep 15 .

PubMed
[Influenza A H1N1 in neonatal intensive care unit: analysis and lessons].
[Influenza A H1N1 in neonatal intensive care unit: analysis and lessons].
Arch Pediatr. 2011 Oct ;18(10):1069-75. Epub 2011 Sep 15 .

PubMed
Shunt-type and stop-type varicocele in adolescents: prognostic value of these tw...
Shunt-type and stop-type varicocele in adolescents: prognostic value of these two different hemodynamic patterns.
Fertil Steril. 2011 Nov ;96(5):1091-6. Epub 2011 Sep 15 .

PubMed
[Screening of low-birthweight babies with calf circumference measurement. Study ...
[Screening of low-birthweight babies with calf circumference measurement. Study of a newborn population in the Abass Ndao Hospital Center of Dakar (Senegal)].
Arch Pediatr. 2011 Oct ;18(10):1113-4. Epub 2011 Sep 15 .

PubMed
Cost of disorders of the brain in Europe 2010.
Cost of disorders of the brain in Europe 2010.
Eur Neuropsychopharmacol. 2011 Oct ;21(10):718-79. Epub 2011 Sep 15 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: