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Nat Prod Res. 2012;26(20):1939-41. Epub 2011 Sep 19.

Simultaneous estimation of hydroxychavicol and chlorogenic acid from Piper betel L. through RP-HPLC.

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  • 1Department of Pharmaceutical Technology, School of Natural Product Studies, Jadavpur University, Kolkata 700 032, India.

Abstract

A RP-HPLC method was developed (λ (max) =280) to quantify hydroxychavicol and chlorogenic acid in Piper betel Linn. The method was validated for linearity, limit of detection (LOD=3:1σ/S), limit of quantification (LOQ=10:1σ/S), precision, accuracy and ruggedness. The response was linear with good correlation between concentration and mean peak area through a coefficient of determinants (r (2)) of 0.9940, y=1.98e +004x +5.19e +004 and 0.9945, y=2.76e+004x+1.40e+005 with LOD 1.6 µg mL(-1), 1.0 µg mL(-1) and LOQ 5.0 µg mL(-1) and 3.0 µg mL(-1), respectively, for hydroxychavicol (28.56% w/w) and chlorogenic acid (0.40% w/w). The %RSD of precision and recovery of hydroxychavicol and chlorogenic acid were <2.0%. The proposed method was simple, accurate, specific, precise and reproducible.

PMID:
21923622
[PubMed - indexed for MEDLINE]
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