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Science. 2011 Sep 16;333(6049):1637-40. doi: 10.1126/science.1207699.

Yeast Rrn7 and human TAF1B are TFIIB-related RNA polymerase I general transcription factors.

Author information

  • 1Fred Hutchinson Cancer Research Center, Division of Basic Sciences, 1100 Fairview Avenue N, Post Office Box 19024, Mailstop A1-162, Seattle, WA 98109, USA.

Abstract

Eukaryotic and archaeal multisubunit RNA polymerases (Pols) are structurally related and require several similar components for transcription initiation. However, none of the Pol I factors were known to share homology with transcription factor IIB (TFIIB) or TFIIB-related proteins, key factors in the initiation mechanisms of the other Pols. Here we show that Rrn7, a subunit of the yeast Pol I core factor, and its human ortholog TAF1B are TFIIB-like factors. Although distantly related, Rrn7 shares many activities associated with TFIIB-like factors. Domain swaps between TFIIB-related factors show that Rrn7 is most closely related to the Pol III general factor Brf1. Our results point to the conservation of initiation mechanisms among multisubunit Pols and reveal a key function of yeast core factor/human SL1 in Pol I transcription.

PMID:
21921198
[PubMed - indexed for MEDLINE]
PMCID:
PMC3319074
Free PMC Article

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