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Mol Biosyst. 2011 Nov;7(11):3152-6. doi: 10.1039/c1mb05270e. Epub 2011 Sep 14.

A photocrosslinking assay for reporting protein interactions in polyketide and fatty acid synthases.

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  • 1Department of Chemistry, North Carolina State University, Raleigh, NC 27695 USA.


Understanding protein-protein interactions that occur between ACP and KS domains of polyketide synthases and fatty acid synthases is critical to improving the scope and efficiency of combinatorial biosynthesis efforts aimed at producing non-natural polyketides. Here, we report a facile strategy for rapidly reporting such ACP-KS interactions based on the incorporation of an amino acid with photocrosslinking functionality. Crucially, this photocrosslinking strategy can be applied to any polyketide or fatty acid synthase regardless of substrate specificity, and can be adapted to a high-throughput format for directed evolution studies.

This journal is © The Royal Society of Chemistry 2011

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