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Curr Opin Genet Dev. 2011 Oct;21(5):538-48. doi: 10.1016/j.gde.2011.08.003. Epub 2011 Sep 13.

Advances in multiphoton microscopy for imaging embryos.

Author information

  • 1Laboratory for Optics and Bioscience, Ecole Polytechnique ParisTech, CNRS, and INSERM U696, Palaiseau, France. willy.supatto@polytechnique.edu

Abstract

Multiphoton imaging is a promising approach for addressing current issues in systems biology and high-content investigation of embryonic development. Recent advances in multiphoton microscopy, including light-sheet illumination, optimized laser scanning, adaptive and label-free strategies, open new opportunities for embryo imaging. However, the literature is often unclear about which microscopy technique is most adapted for achieving specific experimental goals. In this review, we describe and discuss the key concepts of imaging speed, imaging depth, photodamage, and nonlinear contrast mechanisms in the context of recent advances in live embryo imaging. We illustrate the potentials of these new imaging approaches with a selection of recent applications in developmental biology.

Copyright © 2011 Elsevier Ltd. All rights reserved.

PMID:
21917444
[PubMed - indexed for MEDLINE]
PMCID:
PMC3241964
Free PMC Article

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