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Mol Imaging Biol. 2012 Aug;14(4):480-8. doi: 10.1007/s11307-011-0518-y.

Orthotopic expression of noggin protein in cancer cells inhibits human lung carcinoma growth in vivo.

Author information

  • 1Laboratory of Molecular Imaging Probes, Department of Radiology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA, USA.

Abstract

PURPOSE:

We explored the effect of Noggin protein expression on tumor growth in vivo by using fluorescence imaging.

PROCEDURES:

Human lung carcinoma MV522 cells were transduced by using bicistronic (EGFP/Nog) or a control (EGFP) lentivirus at >95% efficacy. The transduced cells were implanted in athymic mice either individually or after mixing with DsRed2-expressing MV522 cells.

RESULTS:

The expression of Noggin protein was demonstrated in EGFP+/Nog+ but not in EGFP+ cell lysates and conditioned media. Noggin did not inhibit tumor cell proliferation in vitro. Implantation of EGFP+ resulted in rapid tumor growth, whereas mice implanted with EGFP+/Nog+ either failed to develop tumors or developed smaller slowly proliferating ones. In the case of tumors grown from mixtures with DsRed2+ cells, only Noggin-expressing cells resulted in decreased tumor volumes with low vascular density and poorly developed stroma.

CONCLUSION:

The effect of Noggin protein expression is a consequence of inhibition of stromal and/or endothelial proliferation in vivo.

PMID:
21913026
[PubMed - indexed for MEDLINE]
PMCID:
PMC4086942
Free PMC Article

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