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Mol Imaging Biol. 2012 Aug;14(4):480-8. doi: 10.1007/s11307-011-0518-y.

Orthotopic expression of noggin protein in cancer cells inhibits human lung carcinoma growth in vivo.

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  • 1Laboratory of Molecular Imaging Probes, Department of Radiology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA, USA.



We explored the effect of Noggin protein expression on tumor growth in vivo by using fluorescence imaging.


Human lung carcinoma MV522 cells were transduced by using bicistronic (EGFP/Nog) or a control (EGFP) lentivirus at >95% efficacy. The transduced cells were implanted in athymic mice either individually or after mixing with DsRed2-expressing MV522 cells.


The expression of Noggin protein was demonstrated in EGFP+/Nog+ but not in EGFP+ cell lysates and conditioned media. Noggin did not inhibit tumor cell proliferation in vitro. Implantation of EGFP+ resulted in rapid tumor growth, whereas mice implanted with EGFP+/Nog+ either failed to develop tumors or developed smaller slowly proliferating ones. In the case of tumors grown from mixtures with DsRed2+ cells, only Noggin-expressing cells resulted in decreased tumor volumes with low vascular density and poorly developed stroma.


The effect of Noggin protein expression is a consequence of inhibition of stromal and/or endothelial proliferation in vivo.

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