Abstract

The amino acid sequence of a glycolipid transfer protein from pig brain was determined by automatic sequencing and fast atom bombardment mass spectroscopic analysis of peptides produced by chemical and enzymatic cleavage reactions. The protein consists of 208 residues, with N-acetylalanine as the N-terminal residue and valine as the C-terminal residue. It contains 3 cysteine residues. The primary structure of the glycolipid transfer protein from pig brain is as follows: acetyl-A-L-L-A-E-H-L-L-K-P-L-P-A-D-K15-Q-I-E-T- G-P-F-L-E-A-V-S-H-L-P30-P-F-F-D-C-L-G-S-P-V-F- T-P-I-K45-A-D-I-S-G-N-I-T-K-I-K-A-V-Y-D60-T-N- P-A-K-F-R-T-L-Q-N-I-L-E-V75-E-K-E-M-Y-G-A-E- W-P-K-V-G-A-T90-L-A-L-M-W-L-K-R-G-L-R-F-I-Q- V105-F-L-Q-S-I-C-D-G-E-R-D-E-N-H-P120-N-L-I-R- V-N-A-T-K-A-Y-E-M-A-L135-K-K-Y-H-G-W-I-V-Q- K-I-F-Q-A-A150-L-Y-A-A-P-Y-K-S-D-F-L-K-A-L- S165-K-G-Q-N-V-T-E-E-E-C-L-E-K-V-R180-L-F-L-V- N-Y-T-A-T-I-D-V-I-Y-E195-M-Y-T-K-M-N-A-E-L-N- Y-K-V-OH. The sequence does not have detectable homology with other lipid transfer proteins or lipid-binding proteins. The cysteine residue at position 35 is reactive to iodoacetamide under nondenaturing conditions.