Format

Send to:

Choose Destination
See comment in PubMed Commons below

[Preparation and identification of OmpW monoclonal antibodies].

[Article in Chinese]

Author information

  • 1Institute of Pharmaceutical Biotechnology and Bioengineering, Fuzhou University, Fuzhou 350002, China. zhenningma2008@yahoo.cn

Abstract

AIM:

To prepare and characterize the mouse monoclonal antibodies against Vibrio parahaemolyticus OmpW.

METHODS:

The OmpW amino acid sequence from three diseased Vibrio was analyzed by Bioinformatics. Mice were immunized with r-OmpW which was highly expressed and purified in E.coli. Five Vibrio(Va, Vp, Vh, Vv, Van) were chosen as antigen for mAb selection.The characters of the anti-OmpW monoclonal antibodies were studied by Western blot, Flow Cytometry, indirect immunofluorescence.

RESULTS:

OmpW was testified a highly conservative membrane protein.Three clones of anti-OmpW mAb was obtained. The Ig subclass of the mAb secreted from fused cell S5C10 was IgG3, which of the titer was 4.6×10(4);. The mAb could specifically recognize Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi, Vibrio anguillarum, Vibrio vulnificus, which could not react with Pseudomonas flurosecens, Aeromonas hydrophila, Aeromonas sobria, Aeromonas hydrophila, Escherichia coli.

CONCLUSION:

The mAb could specially recognize five diseased Vibrio, which is a useful tool for the further study of the diagnosis of Vibrio.

PMID:
21906472
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk