Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Bioelectrochemistry. 2012 Feb;83:25-30. doi: 10.1016/j.bioelechem.2011.08.001. Epub 2011 Aug 18.

Novel conductometric biosensor based on three-enzyme system for selective determination of heavy metal ions.

Author information

  • 1Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine. alex_sold@yahoo.com

Abstract

A differential pair of planar thin-film interdigitated electrodes, deposited on a ceramic pad, was used as a conductometric transducer. The three-enzyme system (invertase, mutarotase, glucose oxidase), immobilized on the transducer surface, was used as a bioselective element. The ratio between enzymes in the membrane was found experimentally considering the highest biosensor sensitivity to substrate (sucrose) and heavy metal ions. Optimal concentration of sucrose for inhibitory analysis was 1.25 mM and incubation time in the investigated solution amounted to 10-20 min. The developed biosensor demonstrated the best sensitivity toward ions Hg(2+) and Ag(+). A principal possibility of the biosensor reactivation either by EDTA solution after inhibition with silver ions or by cysteine solution after inhibition with mercury ions was shown.

Copyright © 2011 Elsevier B.V. All rights reserved.

PMID:
21903487
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk