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Methods Mol Biol. 2011;785:183-9. doi: 10.1007/978-1-61779-286-1_12.

Assessment of antibody specificity using suspension bead arrays.

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  • 1SciLifeLab Stockholm, KTH - Royal Institute of Technology, Tomtebodav, Sweden.

Abstract

With the increasing collection of affinity reagents, their validation in terms of functionality and binding specificity becomes a challenge. To match this growing need, miniaturized and parallelized platforms have become available to corroborate the applicability for a broad range of binder scaffolds. Among the -commonly used systems, planar microarrays have been a platform of choice for a long time but alternative systems are emerging, of which one is based on color-coded beads for the creation of arrays in suspension. The latter systems offer to perform a two-dimensional multiplexing by now analyzing up to 384 samples against up to 500 analytes in a single experiment. While the analyte parameter is flexible in terms of its composition, an extended screening can be facilitated without the need to set up a microarray production facility.

PMID:
21901600
[PubMed - indexed for MEDLINE]
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