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Clin Lab. 2011;57(7-8):631-4.

Presence of murine leukemia virus (MLV)-related virus gene sequences in a commercial RT-PCR reagent.

Author information

  • 1Medical Laboratory Bremen, Bremen, Germany. dietmar.wolff@mlhb.de

Abstract

BACKGROUND:

The recent identification of murine leukemia virus (MLV)-related viruses in patients with chronic fatigue syndrome (CFS) has aroused much interest, not least among sufferers. However, other studies failed to detect these viruses in CFS patients.

METHODS:

We wanted to establish a MLV-related virus real-time PCR for routine diagnostics.

RESULTS:

Our study identified false positive MLV-related virus results due to a contamination of Superscript III Platinum One-Step Quantitative RT-PCR System (Invitrogen).

CONCLUSIONS:

This observation may be helpful to elucidate discrepant results for the detection of MLV-related virus like xenotropic MLV-related virus (XMRV) in recently published studies.

PMID:
21888029
[PubMed - indexed for MEDLINE]
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