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J Pediatr Neurosci. 2009 Jul;4(2):76-85. doi: 10.4103/1817-1745.57326.

Adhesion molecule levels in serum and cerebrospinal fluid in children with bacterial meningitis and sepsis.

Author information

  • 1Department of Pediatrics, Faculty of Medicine, King Abdelaziz University, Jeddah, Saudi Arabia.

Abstract

BACKGROUND:

Adhesion molecules play a role in leukocyte recruitment during central nervous system (CNS) inflammation.

AIM:

This study was designed to compare serum, cerebrospinal fluid (CSF) concentrations of adhesion molecules in children with meningitis and sepsis, and to evaluate their sources.

SETTING:

This study was carried out at Pediatric Department, King Abdulaziz University Hospital from January 2007 to June 2008.

DESIGN:

Serum and CSF samples were collected on admission from meningitis (n = 40), sepsis (n = 20) patients, and sera from controls (n = 20).

MATERIALS AND METHODS:

Endothelial (E), leukocyte (L), platelet (P) selectins intercellular cell adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecules-1 (VCAM-1) were measured using ELISA.

STATISTICS:

ANOVA and Spearman's correlations were used. Adhesion molecules with albumin concentration were estimated in CSF/serum to calculate concentration quotients.

RESULTS:

In meningitis, serum sE-, sL-, sP-selectins sICAM-1, sVCAM-1 levels were higher than controls. Compared to sepsis, serum sE-selectin, sL-selectin, sVCAM-1, CSF-sL-selectin, CSF-sVCAM-1, VCAM-1 ratio and index were higher, while serum sP-selectin was lower than meningitis. sE-selectin ratio, CSF sICAM-1 were higher in meningitis with positive than negative culture. The sE-selectin index was higher in meningitis with neurological complication than those without it. In meningitis, correlation was found between CSF protein and CSF white blood cell counts (WBCs), CSF sICAM-1, CSF sVCAM-1 and between CSF sE-selectin and CSF sICAM-1.

CONCLUSIONS:

This study supports the role of adhesion molecules especially sL-selectin, sVCAM-1 in meningitis and suggests further research to determine their use as biomarkers for meningitis and use of their antagonists as therapeutic for CNS inflammation. The presence of discrepancy of CSF/serum ratios for molecules of same molecular weight suggest intrathecal shedding in addition to diffusion through the blood-CSF barrier.

KEYWORDS:

Adhesion molecules; children; immunoglobulin family; meningitis; selectin; sepsis

PMID:
21887188
[PubMed]
PMCID:
PMC3162794
Free PMC Article
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